Thiophosphorylation of free amino acids and enzyme protein by thiophosphoramidate ions

…, T Frączyk, J Cieśla, A Leś, Z Szewczuk, W Rode

Index: Ruman, Tomasz; Dlugopolska, Karolina; Jurkiewicz, Agata; Rut, Dagmara; Fraczyk, Tomasz; Ciesla, Joanna; Les, Andrzej; Szewczuk, Zbigniew; Rode, Wojciech Bioorganic Chemistry, 2010 , vol. 38, # 2 p. 74 - 80

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Citation Number: 16

Abstract

In search of an activity-preserving protein thiophosphorylation method, with thymidylate synthase recombinant protein used as a substrate, potassium thiophosphoramidate and diammonium thiophosphoramidate salts in Tris-and ammonium carbonate based buffer solutions were employed, proving to serve as a non-destructive environment. Using potassium phosphoramidate or diammonium thiophosphoramidate, a series of ...