Relationship between the Hydrophobicity of Dipeptides and the Michaelis-Menten Constant Km of Their Hydrolysis by Carboxypeptidase-Y and Carboxypeptidase-A
Y Kanosue, S Kojima, Y Hiraga, K Ohkata
Index: Kanosue, Yoshifumi; Kojima, Satoshi; Hiraga, Yoshikazu; Ohkata, Katsuo Bulletin of the Chemical Society of Japan, 2004 , vol. 77, # 6 p. 1187 - 1193
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Abstract
The enzymatic hydrolysis of dipeptides by carboxypeptidase-Y and carboxypeptidase-A was investigated. In the enzymatic hydrolysis of the dipeptides, a good linear relationship (r= 0.997 and 0.999) was found between the Michaelis–Menten constant (K m) and the hydrophobicity of the substrates evaluated from relative elution volume in reversed-phase HPLC. The correlation suggests that the hydrophobicity of the C-terminal amino acid is a ...
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