Influence of Substrate Structure on PGA??Catalyzed Acylations. Evaluation of Different Approaches for the Enzymatic Synthesis of Cefonicid

…, S Rocchietti, R Fernández??Lafuente…

Index: Terreni, Marco; Tchamkam, Joseph Gapesie; Sarnataro, Umberto; Rocchietti, Silvia; Fernandez-Lafuente, Roberto; Guisan, Jose M. Advanced Synthesis and Catalysis, 2005 , vol. 347, # 1 p. 121 - 128

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Citation Number: 16

Abstract

Abstract The influence of the substrate structure on the catalytic properties of penicillin G acylase (PGA) from Escherichia coli in kinetically controlled acylations has been studied. In particular, the affinity of different β-lactam nuclei towards the active site has been evaluated considering the ratio between the rate of synthesis (vs) and the rate of hydrolysis of the acylating ester (v h1). 7-Aminocephalosporanic acid (7-ACA) and 7-amino-3-(1- ...