Continuous??Flow Reactor??Based Enzymatic Synthesis of Phosphorylated Compounds on a Large Scale

L Babich, AF Hartog, MA van der Horst…

Index: Babich, Lara; Hartog, Aloysius F.; Van Der Horst, Michael A.; Wever, Ron Chemistry - A European Journal, 2012 , vol. 18, # 21 p. 6604 - 6609

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Citation Number: 27

Abstract

Abstract Acid phosphatase, an enzyme that is able to catalyze the transfer of a phosphate group from cheap pyrophosphate to alcoholic substrates, was covalently immobilized on polymethacrylate beads with an epoxy linker (Immobeads-150 or Sepabeads EC-EP). After immobilization 70% of the activity was retained and the immobilized enzyme was stable for many months. With the immobilized enzyme we were able to produce and prepare D- ...