Bioorganic & medicinal chemistry

Functional characterization of recombinant hyoscyamine 6β-hydroxylase from Atropa belladonna

J Li, MJ van Belkum, JC Vederas

Index: Li, Jing; Van Belkum, Marco J.; Vederas, John C. Bioorganic and Medicinal Chemistry, 2012 , vol. 20, # 14 p. 4356 - 4363

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Citation Number: 15

Abstract

(−)-Hyoscyamine, the enantiomerically pure form of atropine, and its derivative scopolamine are tropane alkaloids that are extensively used in medicine. Hyoscyamine 6β-hydroxylase (H6H, EC 1.14. 11.11), a monomeric α-ketoglutarate dependent dioxygenase, converts (−)- hyoscyamine to its 6, 7-epoxy derivative, scopolamine, in two sequential steps. In this study, H6H of Atropa belladonna (AbH6H) was cloned, heterologously expressed in Escherichia ...