Substrate profile of an ω-transaminase from Burkholderia vietnamiensis and its potential for the production of optically pure amines and unnatural amino acids
J Jiang, X Chen, J Feng, Q Wu, D Zhu
Index: Jiang, Jinju; Chen, Xi; Feng, Jinhui; Wu, Qiaqing; Zhu, Dunming Journal of Molecular Catalysis B: Enzymatic, 2014 , vol. 100, p. 32 - 39
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Abstract
Abstract A new (S)-enantioselective ω-transaminase (ω-TA) gene from Burkholderia vietnamiensis G4 was functionally expressed in Escherichia coli BL21 (DE3), and the purified recombinant N-terminal His-tagged ω-TA (HBV-ω-TA) had a dimeric structure with optimum pH and temperature of 8.4 and 40 C, respectively. The enzyme showed higher activities toward aromatic amines than aliphatic amines and (S)-1-methylbenzylamine ((S) ...
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