Rigid, conjugated, fluoresceinated thymidine triphosphates: syntheses and polymerase mediated incorporation into DNA analogues
…, WC Haaland, ML Metzker, K Burgess
Index: Thoresen, Lars H.; Jiao, Guan-Sheng; Haaland, Wade C.; Metzker, Michael L.; Burgess, Kevin Chemistry - A European Journal, 2003 , vol. 9, # 19 p. 4603 - 4610
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Citation Number: 101
Abstract
Abstract Syntheses of a unique set of energy transfer dye labeled nucleoside triphosphates, compounds 1–3, are described. Attempts to prepare these compounds were only successful if the triphosphorylation reaction was performed before coupling the dye to the nucleobase, and not the other way around. Compounds were prepared as both the 2′-deoxy (a) and 2′, 3′-dideoxy-(b) forms. They feature progressively longer rigid conjugated linkers ...
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