Engineered dehydrogenase biocatalysts for non-natural amino acids: efficient isolation of the D-enantiomer from racemic mixtures

F Paradisi, PA Conway, AR Maguire…

Index: Paradisi, Francesca; Conway, Philip A.; Maguire, Anita R.; Engel, Paul C. Organic and Biomolecular Chemistry, 2008 , vol. 6, # 19 p. 3611 - 3615

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Citation Number: 14

Abstract

With a view to their use in the kinetic resolution of racemic non-natural amino acids, five variants of the enzyme L-phenylalanine dehydrogenase, the wild-type enzyme from Bacillus sphaericus and four active-site mutants, have been tested with a range of amino acids. In each case, the rates of reaction with 0.2 mM L-amino acid and with the racemic mixture at 0.4 mM were compared, so that the starting concentration of the active substrate was kept ...