A novel protein crosslinking reagent for the determination of moderate resolution protein structures by mass spectrometry (MS3-D)

N Fujii, RB Jacobsen, NL Wood, JS Schoeniger…

Index: Fujii, Naoaki; Jacobsen, Richard B.; Wood, Nichole L.; Schoeniger, Joseph S.; Guy, R. Kiplin Bioorganic and Medicinal Chemistry Letters, 2004 , vol. 14, # 2 p. 427 - 429

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Citation Number: 25

Abstract

A new approach to the determination of moderate resolution protein structures, termed MS3- D—Mass Spectrometry in 3 Dimensions—has recently been disclosed. The method involves the formation of covalent crosslinks between reactive residues on the protein surface, the determination of the location of those crosslinks in primary sequence space by mass spectrometry, and then the imposition of a distance constraint upon the location of the ...