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Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding
10.1126/science.aam8999 2018-04-13 To facilitate scalable profiling of single cells, we developed split-pool ligation-based transcriptome sequencing (SPLiT-seq), a single-cell RNA-seq (scRNA-seq) method that labels the cellular origin of RNA through combinatorial barcoding. SPLiT-seq is compat... |
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Structural basis for coupling protein transport and N-glycosylation at the mammalian endoplasmic reticulum
10.1126/science.aar7899 2018-04-13 Protein synthesis, transport, and N-glycosylation are coupled at the mammalian endoplasmic reticulum by complex formation of a ribosome, the Sec61 protein-conducting channel, and oligosaccharyltransferase (OST). Here we used different cryo–electron microscopy... |
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Structure of the nuclear exosome captured on a maturing preribosome
10.1126/science.aar5428 2018-04-13 The RNA exosome complex processes and degrades a wide range of transcripts, including ribosomal RNAs (rRNAs). We used cryo–electron microscopy to visualize the yeast nuclear exosome holocomplex captured on a precursor large ribosomal subunit (pre-60S) during ... |
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Photoperiodic control of seasonal growth is mediated by ABA acting on cell-cell communication
10.1126/science.aan8576 2018-04-13 In temperate and boreal ecosystems, seasonal cycles of growth and dormancy allow perennial plants to adapt to winter conditions. We show, in hybrid aspen trees, that photoperiodic regulation of dormancy is mechanistically distinct from autumnal growth cessati... |
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Observation of topological superconductivity on the surface of an iron-based superconductor
10.1126/science.aan4596 2018-04-13 Topological superconductors are predicted to host exotic Majorana states that obey non-Abelian statistics and can be used to implement a topological quantum computer. Most of the proposed topological superconductors are realized in difficult-to-fabricate hete... |
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Quantitative analysis of population-scale family trees with millions of relatives
10.1126/science.aam9309 2018-04-13 Family trees have vast applications in fields as diverse as genetics, anthropology, and economics. However, the collection of extended family trees is tedious and usually relies on resources with limited geographical scope and complex data usage restrictions.... |
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Predicting reaction performance in C–N cross-coupling using machine learning
10.1126/science.aar5169 2018-04-13 Machine learning methods are becoming integral to scientific inquiry in numerous disciplines. We demonstrated that machine learning can be used to predict the performance of a synthetic reaction in multidimensional chemical space using data obtained via high-... |
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Rewritable multi-event analog recording in bacterial and mammalian cells
10.1126/science.aap8992 2018-04-13 We present two CRISPR-mediated analog multi-event recording apparatus (CAMERA) systems that use base editors and Cas9 nucleases to record cellular events in bacteria and mammalian cells. The devices record signal amplitude or duration as changes in the ratio ... |
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Science for state legislatures
10.1126/science.aat7661 2018-04-06 Next week on 14 April, the March for Science will once again demonstrate the widespread public support for science around the world. Nowhere is this message more needed than in Washington, D.C., where the federal government continues to ignore the value of in... |
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News at a glance
10.1126/science.360.6384.10 2018-04-06 In science news around the world, U.S. President Donald Trump's administration proposes to roll back tough auto mileage standards championed by former President Barack Obama to combat climate change. French President Emmanuel Macron announces a plan to turn h... |