Latest Articles of Bioconjugate Chemistry


p-maleimidophenyl isocyanate: a novel heterobifunctional linker for hydroxyl to thiol coupling. Bioconjug. Chem. 4 , 212, (1993) 1993-01-01 p-Maleimidophenyl isocyanate (PMPI, 1) is a heterobifunctional cross-linking agent useful for thiol to hydroxyl coupling. Several maleimide-activated compounds were prepared and characterized and then shown to be reactive with thiol-containing proteins. Examp...
Peroxidase-linked anti-basic fibroblast growth factor monoclonal antibody Fab' conjugates: application for two-site enzyme immunoassay and immunohistochemical detection. Bioconjug. Chem. 4(2) , 134-8, (1993) 1993-01-01 After conjugating thiol groups in the hinge region of monoclonal antibody (mAb) Fab' fragments specific for basic fibroblast growth factor (bFGF) with maleimido-horseradish peroxidase HRP) complexes synthesized by incubation of HRP with the heterobifunctional...
Synthesis and radioiodination of N-Boc-p-(tri-n-butylstannyl)-L-phenylalanine tetrafluorophenyl ester: preparation of a radiolabeled phenylalanine derivative for peptide synthesis. Bioconjug. Chem. 4(6) , 574-80, (1993) 1993-01-01 An investigation to prepare a phenylalanine derivative which could be radioiodinated and used directly in peptide synthesis was conducted. N-Boc-p-(tri-n-butylstannyl)-L-phenylalanine tetrafluorophenyl ester was targeted and synthesized from N-Boc-p-iodo-L-ph...
Synthesis of 2-(p-thiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid: application of the 4-methoxy-2,3,6-trimethylbenzenesulfonamide protecting group in the synthesis of macrocyclic polyamines. Bioconjug. Chem. 4(3) , 236-45, (1993) 1993-01-01 A synthesis of the bifunctional chelator 2-(p-thiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid [2-(p-NCS-Bz)-NOTA] is described which illustrates the especial utility of the (4-methoxy-2,3,6-trimethylphenyl)sulfonyl (Mtr) protecting group as an...
Preparation of sulfhydrylborane-dextran conjugates for boron neutron capture therapy. Bioconjug. Chem. 4(6) , 570-3, (1993) 1993-01-01 This study presents a carrier system for boron, potentially useful in boron neutron capture therapy (BNCT). Na2B12H11SH (BSH) was covalently coupled to dextran derivatives. This was accomplished in two ways. The first method comprises activation of dextran wi...
Labeling of immunoglobulins with bifunctional, sulfhydryl-selective, and photoreactive coumarins. Bioconjug. Chem. 4(4) , 300-4, (1993) 1993-01-01 4-(Bromomethyl)-6,7-dimethoxy-2-oxo-2H-benzopyran (1a) and 4-(bromomethyl)-7-methoxy-2-oxo-2H-benzopyran (1b) and their iodo analogs (2) reacted selectively with the sulfhydryl groups generated in a limited reduction of the hinge disulfide bonds of an immunog...
Protected thiol-polyethylene glycol: a new activated polymer for reversible protein modification. Bioconjug. Chem. 4(5) , 314-8, (1993) 1993-01-01 Monomethoxypolyethylene glycol (mPEG) of average molecular weight 5000 was transformed in a series of synthetic steps to a new activated form of PEG, a stable thiol-protected intermediary, for reaction with cysteine residues in proteins under mild conditions ...
A non-nucleotide-based linking method for the preparation of psoralen-derivatized methylphosphonate oligonucleotides. Bioconjug. Chem. 3(5) , 366-74, (1992) 1992-01-01 A method is reported for conjugating an analog of 4'-(aminomethyl)-4,5',8- trimethylpsoralen to methylphophonate oligonucleotides. This method enables the psoralen moiety to be coupled to the phosphonate backbone between any two desired bases in a sequence. W...
Introduction of aliphatic amino and hydroxy groups to keto steroids using O-substituted hydroxylamines. Bioconjug. Chem. 3(2) , 182-6, (1992) 1992-01-01 (Aminooxy)butyl- and -hexylamines and alcohols were synthesized by the Ing-Manske modification of the Gabriel synthesis. The aminooxy group of these heterobifunctional spacer reagents is a far more powerful nucleophile than the amino or hydroxy group because ...
Studies of the influence of different cross-linking reagents on the immune response against a B-epitope. Bioconjug. Chem. 3(1) , 80-4, (1992) 1992-01-01 We have previously shown that the carrier polytuftsin obtained by polycondensation of tuftsin, a naturally occurring macrophage activator, increases significantly the antibody response against a linked B-epitope. In the present work, we have studied the influ...

p-maleimidophenyl isocyanate: a novel heterobifunctional linker for hydroxyl to thiol coupling.

Bioconjug. Chem. 4 , 212, (1993)

1993-01-01

p-Maleimidophenyl isocyanate (PMPI, 1) is a heterobifunctional cross-linking agent useful for thiol to hydroxyl coupling. Several maleimide-activated compounds were prepared and characterized and then shown to be reactive with thiol-containing proteins. Examp...

Peroxidase-linked anti-basic fibroblast growth factor monoclonal antibody Fab' conjugates: application for two-site enzyme immunoassay and immunohistochemical detection.

Bioconjug. Chem. 4(2) , 134-8, (1993)

1993-01-01

After conjugating thiol groups in the hinge region of monoclonal antibody (mAb) Fab' fragments specific for basic fibroblast growth factor (bFGF) with maleimido-horseradish peroxidase HRP) complexes synthesized by incubation of HRP with the heterobifunctional...

Synthesis and radioiodination of N-Boc-p-(tri-n-butylstannyl)-L-phenylalanine tetrafluorophenyl ester: preparation of a radiolabeled phenylalanine derivative for peptide synthesis.

Bioconjug. Chem. 4(6) , 574-80, (1993)

1993-01-01

An investigation to prepare a phenylalanine derivative which could be radioiodinated and used directly in peptide synthesis was conducted. N-Boc-p-(tri-n-butylstannyl)-L-phenylalanine tetrafluorophenyl ester was targeted and synthesized from N-Boc-p-iodo-L-ph...

Synthesis of 2-(p-thiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid: application of the 4-methoxy-2,3,6-trimethylbenzenesulfonamide protecting group in the synthesis of macrocyclic polyamines.

Bioconjug. Chem. 4(3) , 236-45, (1993)

1993-01-01

A synthesis of the bifunctional chelator 2-(p-thiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid [2-(p-NCS-Bz)-NOTA] is described which illustrates the especial utility of the (4-methoxy-2,3,6-trimethylphenyl)sulfonyl (Mtr) protecting group as an...

Preparation of sulfhydrylborane-dextran conjugates for boron neutron capture therapy.

Bioconjug. Chem. 4(6) , 570-3, (1993)

1993-01-01

This study presents a carrier system for boron, potentially useful in boron neutron capture therapy (BNCT). Na2B12H11SH (BSH) was covalently coupled to dextran derivatives. This was accomplished in two ways. The first method comprises activation of dextran wi...

Labeling of immunoglobulins with bifunctional, sulfhydryl-selective, and photoreactive coumarins.

Bioconjug. Chem. 4(4) , 300-4, (1993)

1993-01-01

4-(Bromomethyl)-6,7-dimethoxy-2-oxo-2H-benzopyran (1a) and 4-(bromomethyl)-7-methoxy-2-oxo-2H-benzopyran (1b) and their iodo analogs (2) reacted selectively with the sulfhydryl groups generated in a limited reduction of the hinge disulfide bonds of an immunog...

Protected thiol-polyethylene glycol: a new activated polymer for reversible protein modification.

Bioconjug. Chem. 4(5) , 314-8, (1993)

1993-01-01

Monomethoxypolyethylene glycol (mPEG) of average molecular weight 5000 was transformed in a series of synthetic steps to a new activated form of PEG, a stable thiol-protected intermediary, for reaction with cysteine residues in proteins under mild conditions ...

A non-nucleotide-based linking method for the preparation of psoralen-derivatized methylphosphonate oligonucleotides.

Bioconjug. Chem. 3(5) , 366-74, (1992)

1992-01-01

A method is reported for conjugating an analog of 4'-(aminomethyl)-4,5',8- trimethylpsoralen to methylphophonate oligonucleotides. This method enables the psoralen moiety to be coupled to the phosphonate backbone between any two desired bases in a sequence. W...

Introduction of aliphatic amino and hydroxy groups to keto steroids using O-substituted hydroxylamines.

Bioconjug. Chem. 3(2) , 182-6, (1992)

1992-01-01

(Aminooxy)butyl- and -hexylamines and alcohols were synthesized by the Ing-Manske modification of the Gabriel synthesis. The aminooxy group of these heterobifunctional spacer reagents is a far more powerful nucleophile than the amino or hydroxy group because ...

Studies of the influence of different cross-linking reagents on the immune response against a B-epitope.

Bioconjug. Chem. 3(1) , 80-4, (1992)

1992-01-01

We have previously shown that the carrier polytuftsin obtained by polycondensation of tuftsin, a naturally occurring macrophage activator, increases significantly the antibody response against a linked B-epitope. In the present work, we have studied the influ...