We report herein the development of an efficient fluorescence assay for serine/threonine kinases using a peptide array. Our approach is based on chemical reactions specific to phosphoserine and phosphothreonine residues, that is, base-mediated β-elimination of the phosphate group and subsequent Michael addition of a thiol-containing fluorescent reagent. This procedure enables the covalent introduction of a fluorescent moiety into the ...
![3',6'-bis(dimethylamino)spiro[2-benzofuran-3,9'-xanthene]-1-one Structure](https://image.chemsrc.com/caspic/180/71639-08-2.png)