Analytical Biochemistry 1985-11-15

Separation by high-performance liquid chromatography of (3R)- and (3S)-beta-leucine as diastereomeric derivatives.

D J Aberhart, J A Cotting, H J Lin

Index: Anal. Biochem. 151 , 88, (1985)

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Abstract

For studies on the coenzyme B12-dependent enzyme, leucine-2,3-aminomutase, (3R)- and (3S)-beta-leucines were synthesized. The 10-camphorsulfonamide p-nitrobenzyl esters could be resolved by normal-phase HPLC. A much better separation was obtained by reversed-phase HPLC of the diastereomeric derivatives obtained by treatment of -leucine with Marfey's reagent (N2-(5-fluoro-2,4-dinitrophenyl)-L-alaninamide).