European Journal of Pharmaceutical Sciences 2010-09-11

Induction of hexokinase I expression in normal and diabetic rats by a brassinosteroid isoform.

P Muthuraman, K Srikumar

Index: Eur. J. Pharm. Sci. 41(1) , 1-9, (2010)

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Abstract

This study aimed to investigate the effect of the plant growth regulator 28-homobrassinolide (HB) on the hexokinase I (HK I) enzyme gene expression in the tissues of normal and streptozotocin-induced diabetic rats. Normal and diabetic rats were administered 50 microg of HB for 15 consecutive days. The tissues level of HK I mRNA expression was quantitated by PCR and densitometry analysis, HK I protein expression was quantitated by Western blot and densitometry analysis, localization of HK I was done by immunohistochemistry and HK enzyme activity was determined by coupled enzyme assay. Subchronic treatment of rats with HB enhanced HK I enzyme expression in diabetic rat compared to the control rat. From these experimental evidences, we came to the conclusion that HK I played a vital role in the regulation of blood sugar in streptozotocin-induced diabetic rats. A direct role for hexokinase enzyme activity in the control of diabetes is presented.Copyright (c) 2010 Elsevier B.V. All rights reserved.