Journal of Bacteriology 2004-04-01

Characterization of Cfa1, a monofunctional acyl carrier protein involved in the biosynthesis of the phytotoxin coronatine.

Heather Seidle, Vidhya Rangaswamy, Robin Couch, Carol L Bender, Ronald J Parry

Index: J. Bacteriol. 186 , 2499-2503, (2004)

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Abstract

Cfa1 was overproduced in Escherichia coli and Pseudomonas syringae, and the degree of 4'-phosphopantetheinylation was determined. The malonyl-coenzyme A:acyl carrier protein transacylase (FabD) of P. syringae was overproduced and shown to catalyze malonylation of Cfa1, suggesting that FabD plays a role in coronatine biosynthesis. Highly purified Cfa1 did not exhibit self-malonylation activity.