Rapid Communications in Mass Spectrometry 1996-01-01

Characterization of two new matrices for matrix-assisted laser desorption/ionization mass spectrometry.

J Bai, X Liang, Y H Liu, Y Zhu, D M Lubman

Index: Rapid Commun. Mass Spectrom. 10(7) , 839-44, (1996)

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Abstract

Three structurally related compounds, 4-hydroxy-33-methoxyphenylpyruvic acid (HMPPA), indole-3 pyruvic acid (IPA), and indole-3-glyoxylic acid have been evaluated as matrix-assisted laser desorption/ionization (MALDI) matrices. HMPPA and IPA were found to be effective matrices for MALDI-MS analysis of proteins and peptides and have been characterized in this work. HMPPA was found to be a particularly good matrix for the analysis of proteins and peptides because of its tolerance towards impurities and the resulting sensitivity in MALDI-MS experiments. Analysis of model proteins and peptides, including those in biological fluid, are demonstrated.