Acta Anatomica 1988-01-01

Histochemical detection of horseradish peroxidase activity in the rat by the vibratome-paraplast method.

R S Liem, J T Rokx, J D van Willigen

Index: Acta Anat. (Basel) 132 , 164-168, (1988)

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Abstract

A method is described for the histochemical detection of horseradish peroxidase in Paraplast Plus embedded brain sections. The procedure uses 150-micron-thick Vibratome-cut slices of glutaraldehyde-paraformaldehyde-fixed brain tissue. Tetramethylbenzidine stabilized by diaminobenzidine/cobalt/H2O2 is used as chromogen. The Vibratome-cut slices are dehydrated through a graded series of acetone, cleared in toluol and flat-embedded in Paraplast Plus embedding medium. Serial sections can be cut as thin as 5-7 micron. The method is universal in its application and permits optimal visualization of labeled neurons with great morphological detail at the light-microscopic level.