Journal of Pharmaceutical and Biomedical Analysis 1991-01-01

Potentiality of an organometallic labelled streptavidin--biotin system in metalloimmunoassay.

I Rémy, P Brossier, I Lavastre, J Besançon, C Moïse

Index: J. Pharm. Biomed. Anal. 9(10-12) , 965-7, (1991)

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Abstract

Biotin labelled with a cymantrene moiety (cyclopentadienyl manganese tricarbonyl complex) is described for the first time. Because this metallo-biotin retains full recognition for the specific glycoprotein avidin (or streptavidin), the labelled streptavidin-biotin system is proposed for use in a solid-phase competition-type metalloimmunoassay in which bovine serum albumin (BSA) is used as a model of applications. Atomic absorption spectrometry is utilized for the detection of the cymantrene-labelled biotin.