Guang pu xue yu guang pu fen xi = Guang pu 2002-12-01

[Spectra studies on interaction of bovine serum albumin with acidic chrome blue K in acidic solution].

Ying Yu, Jian Liao, Fa-de Huang

Index: Guang Pu Xue Yu Guang Pu Fen Xi 22(6) , 1067-9, (2002)

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Abstract

This paper investigated the interaction of acidic chrome blue K with bovine serum albumin (BSA). When BSA was added into acidic chrome blue K solution at pH 2.87 HCl-NaAc buffter, bathochromic effect and hypochromicity were observed. With the increase in BSA concentration, the absorption peak at 523 nm decreased. It was considered that the combination of BSA with acidic chrome blue K is due to static electricity forces. The interaction is in accord with model of phase distribution. It was discussed the effect of acidity, concentration of acidic chrome blue K, ion strength to apparent binding constant Kc, binding number n, Sandell constant. The reaction time, surfactant, work curve were studied.