Use of high-performance liquid chromatography in the measurement of in vitro acetylation in man.

J D Baty, R M Lindsay, S Sharp

Index: J. Chromatogr. A. 353 , 329-37, (1986)

Full Text: HTML

Abstract

Liquid chromatographic methods were developed for the study of the in vitro acetylation of the sulphonamide drug sulphamethazine and a series of aniline derivatives. The sensitivity of the methods have allowed data on the activity of the N-acetyltransferase enzyme(s) in man to be obtained. The use of bonded-phase columns with a series of organic mobile phases has been compared with reversed-phase separation of the acetanilide derivatives, produced in the acetylation reaction.