China Journal of Chinese Materia Medica 2002-01-01

[Determination of cirsilineol in Herba Artemisiae scopariae collected in autumn by HPLC].

Qi-wei Zhang, Yong-xin Zhang, Ying Zhang, Guo-gang Liang

Index: Zhongguo Zhong Yao Za Zhi 27(1) , 23-5, (2002)

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Abstract

To establish a quantitative method for determination of cirsilineol in Herba Artemisiae Scopariae collected in Autumn.Sample was extracted with methanol, and cleaned up with polyamide column. ODS column was used with methanol-acetonitrile-0.5% acetic acid solution (24:19:57) as mobile phase. Detection wavelength was 347 nm.Cirsilineol in sample solution was well separated. Linearity of cirsilineol was good (r = 0.9998) in range of 0.1-0.8 microgram. The average recovery was 101.3%, RSD of repeatability was 3.04%.This method can be used for quality control of Herba Artemisiae Scopariae collected in autumn and its preparations.