International Journal for Vitamin and Nutrition Research 1984-01-01

Quantitative and qualitative effects of N10-methylfolate on high-affinity folate binding in human leukocytes.

J Holm, S I Hansen, J Lyngbye

Index: Int. J. Vitam. Nutr. Res. 54(2-3) , 195-7, (1984)

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Abstract

N10-methylfolate acted as a potent competitive inhibitor of high-affinity [3H] folate binding in human leukocytes, while methotrexate had no effect. Furthermore, folate binding changed into a non-cooperative type in the presence of N10-methylfolate. Hence, in qualitative and quantitative respects, the substrate specificity characteristics of leukocyte folate binding resemble those of other high-affinity folate binding systems.