Journal of enzyme inhibition 1995-01-01

Enolase and the arsonomethyl analogue of 2-phosphoglycerate.

S Chawla, H B Dixon

Index: J. Enzym. Inhib. 8(4) , 255-9, (1995)

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Abstract

(RS)-3-Arsono-2-(hydroxymethyl)propionic acid was synthesized by the action of alkaline arsenite on 3-bromo-2-(bromomethyl)propionic acid. It is a substrate for yeast enolase (EC 4.2.1.11) with a Km of 6.5 mM (for 2-phospho-D-glycerate Km = 0.08 mM). The catalytic constant of the enzyme with the arsonomethyl analogue is 230 times lower than with 2-phosphoglycerate.