Molecular BioSystems 2011-07-01

Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis.

Daniel C Propheter, Lara K Mahal

Index: Mol. Biosyst. 7(7) , 2114-7, (2011)

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Abstract

Herein we describe the orientation of GST-tagged lectins on NHS-activated slides via a one-step deposition of the protein and a glutathione (GSH) scaffold. This technology overcomes the need for a premade GSH-surface to orient GST-tagged proteins, enabling us to rapidly expand the analytical capacity of lectin microarrays through addition of oriented lectins, while maintaining lectin diversity.