Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 1991-01-01

Cell-cycle dependent mutation of human lymphoblasts: bromodeoxyuridine and butyl methanesulfonate.

H Hoppe, K M Call, P M Leong, W G Thilly

Index: Mutat. Res. 250(1-2) , 411-21, (1991)

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Abstract

Cells of the human lymphoblast line WI-L2 and its derivative TK-6 were synchronized by centrifugal elutriation and cell-cycle dependent mutation to 6TGR (HPRT) and OUAR (Na+, K+ ATPase) measured. Bromodeoxyuridine induced 6TGR and OUAR mutations within S phase while butylmethyl-sulfonate induced mutation displayed no cell-cycle dependence. The data indicate that centrifugal elutriation is a facile means to obtain a useful degree of synchrony for these cell lines.