Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie 1981-03-02

[Evidence, in vitro, of estrone and estradiol-17 beta 3-sulfoconjugate hydrolysis in the uterus of pregnant guinea pigs].

G L Adessi, T Q Nhuan, C Goutte-Coussieu, M F Jayle

Index: C. R. Seances. Acad. Sci. III. 292(9) , 609-12, (1981)

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Abstract

The metabolism, in vitro, of [6,7-3H]-oestrone-3-sulfate and [6,7-3H]-oestradiol-17beta-3-sulfate by the uteri of pregnant female Guinea-Pigs has been studied using 900 g supernatants. A hydrolysis of the sulfate moiety was observed: 35% of the radioactivity was recovered in the unconjugated fractions after 1 hr. of incubation. Gluruconide oestrogens were also recovered but the rate of synthesis was very low (3.6% after 1 hr.). OEstrone and oestradiol-17beta have been identified in unconjugated, glucuro and sulfo-conjugated fractions after incubation with the tritiated oestrogen sulfates.