Biotechnology Letters 2010-05-01

Fusion expression of cecropin B-like antibacterial peptide in Escherichia coli and preparation of its antiserum.

Fuxian Yu, Jigui Wang, Peng Zhang, Yan Hong, Weiquan Liu

Index: Biotechnol. Lett. 32(5) , 669-73, (2010)

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Abstract

Antibacterial peptides have a broad range of antibacterial properties that makes them highly toxic for expression in Escherichia coli. For prepare an antiserum to detect these peptides, we developed a cecropin B mutant with a green fluorescent protein fusion partner resulting in high expression of a 37 kDa fusion peptide in E. coli with a yield of 7.9 mg/l culture medium after purification on Ni-IDA resin. Guinea pigs when immunized with the fusion peptide produced a specific antiserum which titers in excess of 1:25,600.