Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.] 2001-05-01

Fluorescent staining of subcellular organelles: ER, Golgi complex, and mitochondria.

M Terasaki, L Loew, J Lippincott-Schwartz, K Zaal

Index: Curr. Protoc. Cell Biol. Chapter 4 , Unit 4.4, (2001)

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Abstract

The ability to distinguish and identify specific subcellular compartments is essential to understanding organelle function, biogenesis, and maintenance within cells and to defining protein trafficking pathways. Fluorescent dyes and/or fluorescently labeled lipid derivatives can be used to identify ER, Golgi complex, and mitochondria. Specific conditions for labeling each of these compartments are described.