Analytical Biochemistry 1973-07-01

A method for the assay of chymotrypsin in crude biological materials.

A R Imondi, R P Stradley, E R Butler, R L Wolgemuth

Index: Anal. Biochem. 54 , 199, (1973)

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Abstract

A method suitable for assaying chymotrypsin in crude biological materials such as feces has been described. The procedure employs a water soluble peptide, N-benzoyl- l-tyrosyl-para-aminobenzoate (Na), which is hydrolyzed by chymotrypsin ( K m = 2.9 × 10 −3 M) to yield the TCA-soluble diazotizable aromatic amine.