Journal of Histochemistry & Cytochemistry 2000-07-01

Applications of plastic embedding to electron microscopic immunocytochemistry and in situ hybridization in observations of production and secretion of peptide hormones.

R Y Osamura, Y Itoh, A Matsuno

Index: J. Histochem. Cytochem. 48(7) , 885-91, (2000)

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Abstract

Plastic embedding has been used to localize various antigens in conjunction with immunohistochemistry. Peptide hormones have been among the antigens that have been studied extensively. Recent application of water-soluble plastics such as LR White and Lowicryl has extended the ranges of detectable antigens and enabled the observation of antigen-antigen or mRNA-antigen combinations. This review article deals with technical aspects, procedures, and applications in endocrine cells.