Bioscience, Biotechnology, and Biochemistry 2012-01-01

Immobilization of a thermostable cellobiose 2-epimerase from Rhodothermus marinus JCM9785 and continuous production of epilactose.

Hiroki Sato, Wataru Saburi, Teruyo Ojima, Hidenori Taguchi, Haruhide Mori, Hirokazu Matsui

Index: Biosci. Biotechnol. Biochem. 76(8) , 1584-7, (2012)

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Abstract

Cellobiose 2-epimerase (CE) efficiently forms epilactose which has several beneficial biological functions. A thermostable CE from Rhodothermus marinus was immobilized on Duolite A568 and packed into a column. Lactose (100 g/L) was supplied to the reactor, kept at 50 °C at a space velocity of 8 h(-1). The epilactose concentration of the resulting eluate was 30 g/L, and this was maintained for 13 d.