… novel Cookson??type reagent for enhancing sensitivity and specificity in assessment of infant vitamin D status using liquid chromatography/tandem mass spectrometry

…, M Morohashi, K Yamagata, T Higashi

Index: Ogawa, Shoujiro; Ooki, Satoshi; Morohashi, Misato; Yamagata, Kenichiro; Higashi, Tatsuya Rapid Communications in Mass Spectrometry, 2013 , vol. 27, # 21 p. 2453 - 2460

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Citation Number: 18

Abstract

METHODS A novel Cookson-type reagent, 4-(4'-dimethylaminophenyl)-1, 2, 4-triazoline-3, 5- dione (DAPTAD), was synthesized from 4-dimethylaminobenzoyl chloride. The DAPTAD- derivative of 25 (OH) D 3 was prepared and its LC/ESI-MS/MS behavior was examined. The applicability of the DAPTAD-derivatization in the determination of 25 (OH) D 3 in neonatal DBSs was also examined. RESULTS The derivatization was completed at room ...