Diagnostic Microbiology and Infectious Disease 1989-01-01

Rapid identification of Candida albicans using 4-methylumbelliferyl N-acetyl-beta-galactosaminide.

M T Dalton, D J Haldane, J MacDonald

Index: Diagn. Microbiol. Infect. Dis. 12(6) , 521-3, (1989)

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Abstract

Rapid identification of Candida albicans is performed mainly by the germ-tube test. However, recent reports have suggested that up to 5% of C. albicans species can give false negative results. We describe the use of 4-Methylumbelliferyl N-acetyl-beta-D-galactosaminide (4-MAG) conjugate as an alternative to the germ-tube test. Our results indicate that, in comparison to the germ-tube test, the 4-MAG test has a sensitivity of 100% and a specificity of 92%. Candida tropicalis can give false-positive results, and that a further screening test is required to identify this species. Problems reading end-points were not encountered.