International journal of fertility 1993-01-01

Provocation testing of human sperm motility using energy substrates and activators of the cyclic nucleotide system: II. Studies on sperm from asthenozoospermic subjects.

O Magnus, T Abyholm, I Brekke, K Purvis

Index: Int. J. Fertil. 38 , 123-128, (1993)

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Abstract

To find differences in effect on sperm motility of agents that increase intracellular cAMP: manganese ion, methyl-isobutyl-xanthine (MIX), 2-deoxyadenosine, glucose, and Mn-MIX and Mn-glucose.Nine men with asthenozoospermia vs. fertile donors.Sperm was washed in Hepes-buffered saline, motility tested by laser-Doppler technique.Best activation was obtained with Mn and 2-deoxyadenosine; generally poor response to MIX or glucose.Usually, poor endogenous stimulation of adenylyl cyclase, and probably not limited energy supply, is the cause of impaired motility.