Acta Histochemica 1990-01-01

[Analytical dye studies on the polarization microscopic detection of collagen with Solaminrot 4B. I].

R Schräpler, R Schultka, T Schmidt, W D Hepp

Index: Acta Histochem. 89(2) , 237-44, (1990)

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Abstract

It is known that the birefringency of the collagen fibres is selectively enhanced by staining with Sirius Red. According to experiences, the most authors usually take Sirius Red F3BA as dye. We use the disazo dyestuff Solaminrot 4B produced by VEB Chemiekombinat Bitterfeld. Thus, the present paper is dealing with physicochemical analysis of the dye. We investigated 2 charges of the Solaminrot 4B. The 1st charge contain supplementary substances; the 2nd charge is without ones. It is reported a series of data resulting from chromatographic and spectroscopic investigations.