Research communications in chemical pathology and pharmacology 1976-03-01

Theophylline radioimmunoassay: synthesis of antigen and characterization of antiserum.

C E Cook, M E Twine, M Myers, E Amerson, J A Kepler, G F Taylor

Index: Res. Commun. Chem. Pathol. Pharmacol. 13 , 497, (1976)

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Abstract

Antisera to theophylline (T) have been obtained by immunizing rabbits with a conjugate of 8-(3-carboxypropyl)-1,3-dimethylxanthine and bovine serum albumin. Comparison of 50% displacement values indicated good selectivity for T vs. a number of other xanthine derivatives. An analytical procedure using this antiserum can measure 200 pg of T and direct analysis of 0.1 mug/ml in plasma or 0.02 mug/ml in saliva is feasible.