Biochimica et Biophysica Acta 1980-04-18

The influence of bile salts and bile lipoprotein complex on pancreatic lipase hydrolysis of monomolecular films.

D Lairon, M Charbonnier-Augeire, G Nalbone, J Leonardi, J C Hauton, G Pieroni, F Ferrato, R Verger

Index: Biochim. Biophys. Acta 618(1) , 106-18, (1980)

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Abstract

We report a new technique which allows us to follow the lipolysis of monomolecular films in the presence of bile salts by using a 'zero-order' trough (Verger, R. and de Haas, G.H. (1973) Chem. Phys. Lipids 10, 127). The effects of bile salts, the bile lipoprotein complex and colipase on pancreatic lipase hydrolysis of rac-1,2-didodecanoylglycerol films were studied at different surface pressures. Taking into account previous studies, lipase activity was interpreted as a function of its degree of binding to the bile lipoprotein complex.