Journal of Bioscience and Bioengineering 2011-08-01

Efficient hydrolytic reaction of an acetate ester with fungal lipase in a liquid-liquid interface bioreactor (L-L IBR) using CaCO₃-coated ballooned microsphere.

Shinobu Oda, Hironori Wakui, Shinichi Ohashi

Index: J. Biosci. Bioeng. 112(2) , 151-3, (2011)

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Abstract

In a liquid-liquid interface bioreactor using a CaCO₃-coated ballooned microsphere, 2-ethylhexyl acetate was efficiently hydrolyzed to 2-ethyl-1-hexanol with Absidia coerulea NBRC 4423 compared with using talc-coated or non-coated ballooned microsphere. It was assumed that CaCO₃ brought about stabilization of lipase by Ca²⁺ and maintenance of medium pH.Copyright © 2011 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.