Organic Letters 2008-11-06

Nonclassical SNAPFL analogue as a Cy5 resonance energy transfer partner.

Sung Hoon Kim, Jillian R Gunther, John A Katzenellenbogen

Index: Org. Lett. 10(21) , 4931-4, (2008)

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Abstract

We have synthesized a new SNAPFL analogue (1) that exhibits a large Stokes shift (78 nm) (abs. 542 nm, em. 620 nm) and a good quantum yield. Because of the large overlap between the emission spectrum of 1 and the absorption spectrum of Cy5, 1 functions well as a fluorescence donor to Cy5 and has been used in FRET-based experiments using estrogen receptor site-specifically labeled with Cy5 and a receptor ligand conjugated to SNAPFL.