Journal of chromatography. B, Biomedical sciences and applications 1997-01-24

Use of thiopropyl sepharose for the synthesis of an adsorbent for the affinity chromatography of glutathione S-transferase.

Z Glatz, J Psotová, O Janiczek, K Chroust, T Jowet

Index: J. Chromatogr. B. Biomed. Sci. Appl. 688(2) , 239-43, (1997)

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Abstract

Thiopropyl Sepharose 6B in the 2-thiopyridyl-activated form was used for the reversible immobilisation of reduced glutathione (GSH). The resulting affinity matrix was successfully tested as a sorbent for the partial purification of glutathione S-transferase (GST) from pig kidney. The specific elution of the enzyme was performed with 10 mM GSH in Tris-HCl buffer (pH 7.8), non-specific elution with 20 mM dithiotreitol (DTT) in the same buffer.