Biochimica et Biophysica Acta 1982-09-07

A convenient and rapid method for the complete removal of CoA from butyryl-CoA dehydrogenase.

G Williamson, P C Engel

Index: Biochim. Biophys. Acta 706(2) , 245-8, (1982)

Full Text: HTML

Abstract

The commercially available gel, 2-pyridyl disulphide hydroxypropyl ether-Sepharose (thiopropyl-Sepharose 6B), can be used to remove bound ligand completely from butyryl-CoA dehydrogenase (EC 1.399.2) in two simple operations. The resultant enzyme forms normal complexes with acetoacetyl-CoA and CoA persulphide, contains no bound CoA as determined by the enzymatic assay for CoA, and retains full catalytic activity.