Biosensors and Bioelectronics 2012-05-15

Amperometric biosensor based on diamond paste for the enantioanalysis of L-lysine.

Raluca-Ioana Stefan-van Staden, R'afat Mahmoud Nejem, Jacobus Frederick van Staden, Hassan Y Aboul-Enein

Index: Biosens. Bioelectron. 35(1) , 439-42, (2012)

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Abstract

An amperometric biosensor was proposed for the enantioanalysis of L-lysine. The biosensor is based on the impregnation of L-lysine oxidase in diamond paste. The potential used for the determination of l-lysine was 650 mV. The biosensor exhibited a linear concentration range between 1 and 100 nmol/L with a limit of detection of 4 pmol/L. The selectivity of the biosensor is high over other amino acids, such as L-serine, L-leucine, L-aspartic acid, L-glutamic acid, histamine, glycine. The proposed biosensor can be applied for the determination of L-lysine in serum samples and pharmaceutical compounds.Copyright © 2012 Elsevier B.V. All rights reserved.