Chemical Communications 2003-04-21

A new dark quencher for use in genetic analysis.

Jonathan P May, Lynda J Brown, Ivo Rudloff, Tom Brown

Index: Chem. Commun. (Camb.) (8) , 970-1, (2003)

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Abstract

A novel, long-wavelength, non-fluorescent quencher (LQ), based on 1,4-diaminoanthraquinone, has been incorporated at the 3' and 5'-termini of oligonucleotides. The quencher has been used in Molecular beacons, efficiently quenching the long wavelength fluorophore, Cy5.