ChemPhysChem 2012-08-06

Surface-attached polyhistidine-tag proteins characterized by FTIR difference spectroscopy.

Philipp Pinkerneil, Jörn Güldenhaupt, Klaus Gerwert, Carsten Kötting

Index: ChemPhysChem 13(11) , 2649-53, (2012)

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Abstract

A universal label-free method for the spectroscopic investigation of polyhistidine-tagged proteins is presented. A solid supported lipid bilayer (SSLB, picture) containing nitrilotriacetic-acid-modified lipids is attached on top of a germanium attenuated total reflection crystal by hydrophilic interactions. Any His tag-modified protein can be immobilized and investigated by FTIR spectroscopy.Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.