Selective zinc sensor molecules with various affinities for Zn2+, revealing dynamics and regional distribution of synaptically released Zn2+ in hippocampal slices

K Komatsu, K Kikuchi, H Kojima, Y Urano…

Index: Komatsu, Kensuke; Kikuchi, Kazuya; Kojima, Hirotatsu; Urano, Yasuteru; Nagano, Tetsuo Journal of the American Chemical Society, 2005 , vol. 127, # 29 p. 10197 - 10204

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Citation Number: 295

Abstract

We have developed a series of fluorescent Zn2+ sensor molecules with distinct affinities for Zn2+, because biological Zn2+ concentrations vary over a wide range from sub-nanomolar to millimolar. The new sensors have K d values in the range of 10-8-10-4 M, compared with 2.7 nM for ZnAF-2. They do not fluoresce in the presence of other biologically important metal ions such as calcium or magnesium, and they can detect Zn2+ within 100 ms. In ...