Science 1983-05-06

Radioactive labeling of antibody: a simple and efficient method.

D J Hnatowich, W W Layne, R L Childs, D Lanteigne, M A Davis, T W Griffin, P W Doherty

Index: Science 220 , 613-615, (1983)

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Abstract

A simple and efficient method of covalently coupling the strong chelator diethylenetriaminepentaacetic acid to proteins was developed for radiolabeling immunoglobulin G antibodies. After being coupled and labeled with indium-111, a monoclonal antibody to carcinoembryonic antigen retained its ability to bind to its antigen in vitro and in vivo. In nude mice with a human colorectal xenograft, 41 percent of the injected radioactivity became localized in each gram of xenograft at 24 hours compared with 9 percent for control antibody and 19 percent for radioiodinated antibody to carcinoembryonic antigen.