Photochemical & Photobiological Sciences 2012-03-01

Caged glucosamine-6-phosphate for the light-control of riboswitch activity.

Bernhard Wulffen, Maximilian Clemens Rudolf Buff, Monika Pofahl, Günter Mayer, Alexander Heckel

Index: Photochem. Photobiol. Sci. 11(3) , 489-92, (2012)

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Abstract

We have synthesized a light-activatable ("caged") derivative of glucosamine-6-phosphate (GlcN6P), which only upon irradiation becomes a cofactor for the glmS riboswitch. This glmS riboswitch maintains its activity when embedded in the 3'-untranslated region of eukaryotic mRNA molecules and caged GlcN6P reduces the amount of translated EGFP upon irradiation with light in vitro.