Electrophoresis 1998-09-01

Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection.

D B Craig, R M Polakowski, E Arriaga, J C Wong, H Ahmadzadeh, C Stathakis, N J Dovichi

Index: Electrophoresis 19 , 2175, (1998)

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Abstract

We report a method for protein labeling, separation by capillary electrophoresis in a polymer sieving matrix, and detection by laser-induced fluorescence. Different dyes are used to label standard and sample proteins. A two-spectral channel detector resolves fluorescence from the sample and standards. Comparison of the migration time of the sample and standards permits the precise determination of molecular weight, irrespective of variations in run-to-run migration times.