To study the chemical constituents of the aerial parts of Seseli mairei Wolf.The chemical constituents have been separated with manifold chromatography methods, and their structures were determined hy spectral analysis.Thirteen compounds were isolated and identified as sphondin (I), hergapten (II), isopimpinellin (III), umbelliferone (IV), chrysosptertin B (V), apiin (VI), rutin (VII), quercetin (VIII), ferulic acid (IX), falcarindiol (X), docosanol (XI), beta-sitosterol (XII), daucosterol (X III).All these compounds were isolated from the aerial parts of Seseli mairei Wolf. for the first time.