Scientific reports 2012-01-01

Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps.

Alice Verchère, Isabelle Broutin, Martin Picard

Index: Sci. Rep. 2 , 306, (2012)

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Abstract

We describe an original activity assay for membrane transport that uses the proton motive force-dependent efflux pump MexAB from Pseudomonas aeruginosa. This pump is co-reconstituted into proteoliposomes together with bacteriorhodopsin (BR), a light-activated proton pump. In this system, upon illumination with visible light, the photo-induced proton gradient created by the BR is shown to be coupled to the active transport of substrates through the pump.